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當前位置：首頁技術文章增殖細胞核抗原（內參）單克隆抗體介紹

增殖細胞核抗原（內參）單克隆抗體介紹

更新時間：2025-07-22點擊次數(shù)：37
產品編號bsm-33035M
英文名稱PCNA Mouse mAb, Nuclear Loading Control
中文名稱增殖細胞核抗原（內參）單克隆抗體
別    名Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; Mutagen-sensitive 209 protein; Pcna/cyclin; PCNAR; Polymerase delta accessory protein; Proliferating Cell Nuclear Antigen
產品類型內參抗體
研究領域腫瘤  細胞生物  細胞周期蛋白  轉錄調節(jié)因子  細胞類型標志物
抗體來源Mouse
克隆類型Monoclonal
克 隆 號10E9
交叉反應Human,Mouse,Rat
產品應用WB=1:500-5000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1:50
           not yet tested in other applications.
           optimal dilutions/concentrations should be determined by the end user.
理論分子量29 kDa
檢測分子量
細胞定位細胞核
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human PCNA
亞    型IgG
純化方法affinity purified by Protein G
緩 沖 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMedPubMed
產品介紹Proliferating cell nuclear antigen (PCNA) is a 28kDa nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular DNA synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic structure.